Documents opposing the ruling were filed at Westminster Magistrates’ Court this morning.
The taxi-app firm had been set a deadline of 4.30pm today to formally appeal or go out of business in the capital.
TfL last month refused it a new licence on the grounds it was not a 'fit and proper' operator.
Around 3.5 million Londoners are said to use Uber. An estimated 40,000 drivers rely on the company for their livelihoods.