In a statement, the retail giant said its Hardwick distribution centre (DC) in Warrington, will close.
In January, M&S said it was closing 14 stores, affecting nearly 500 jobs. The retailer also ditched its Neasden distribution site near London, putting a further 380 jobs at risk - with the official closure scheduled for later this year.
M&S said on Tuesday that it will close its Hardwick distribution centre, which is operated by XPO Logistics and DHL, in September.
The 450staff members affected have entered a consultation period with XPO and DHL.
It said the Hardwick site has become surplus to requirements in part due to M&S's decision to automate operations at its factory in Bradford, which can handle more capacity.
The moves are the latest in the M&S five-year transformation programme which, it claims, "will make M&S a faster, more commercial retailer".
Additionally, M&S has appointed DHL to operate its new South East centre at Welham Green following a competitive tender.
The site is currently being mechanised and fitted to M&S specifications. DHL will operate the site and provide transport operations. It is set to open in 2019 and DHL will recruit around 500 colleagues to work at the site.
Gordon Mowat, director of clothing & home supply chain & logistics, said: "Closing Hardwick will help to remove some complexity from our network and speed up our supply chain.
"However, it was not a decision we took lightly and it is not in any way a reflection on the hard work and dedication provided by the teams on site. We will do all we can to support our partners through the consultation process.
"On our new site at Welham Green, DHL is the right partner for us. They delivered the best response to the tender, they have a strong track record at other M&S sites and are best placed in terms of costs and speed of operation to help us realise the benefits of our new DC.”
The M&S Clothing & Home distribution network currently comprises of 18 sites including large distribution centres in Castle Donington, Bradford and Swindon.
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